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차 의과학대학교 세계적 권위 SCI급 우수 논문 게재 현황

차 의과학대학교에서 세계적 권위가 입증된 SCI(Science Citation Index)급 학술지에 게재된 우수 논문들을 소개합니다.

Generation of human tonsil epithelial organoids as an ex vivo model for SARS-CoV-2 infection

개제 일
2022-03-11
주 저자
유종만(공동교신): 차의과학대학교 의학전문대학원 미생물학교실
공동 저자
최우희: 차의과학대학교 의학전문대학원(연구교수), 배동혁: 차의과학대학교 의학전문대학원(연구교수)
학술지 명
Biomaterials
인용 지수
12.479
Generation of human tonsil epithelial organoids as an ex vivo model for SARS-CoV-2 infection





Han Kyung Kim, Hyeryeon Kim, Myoung Kyu Lee, Woo Hee Choi, Yejin Jang, Jin Soo Shin, Jun-Yeol Park, Dong Hyuck Bae, Seong-In Hyun, Kang Hyun Kim, Hyun Wook Han, Byungho Lim, Gildon Choi, Meehyein Kim, Young Chang Lim, Jongman Yoo


Biomaterials, [Online ahead of print], doi: 10.1016/j.biomaterials.2022.121460


ABSTRACT





The palatine tonsils (hereinafter referred to as "tonsils") serve as a reservoir for viral infections and play roles in the immune system's first line of defense. The aims of this study were to establish tonsil epithelial cell-derived organoids and examine their feasibility as an ex vivo model for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. The tonsil organoids successfully recapitulated the key characteristics of the tonsil epithelium, including cellular composition, histologic properties, and biomarker distribution. Notably, the basal layer cells of the organoids express molecules essential for SARS-CoV-2 entry, such as angiotensin-converting enzyme 2 (ACE2), transmembrane serine protease 2 (TMPRSS2) and furin, being susceptible to the viral infection. Changes in the gene expression profile in tonsil organoids revealed that 395 genes associated with oncostatin M signaling and lipid metabolism were highly upregulated within 72 h after SARS-CoV-2 infection. Notably, remdesivir suppressed the viral RNA copy number in organoid culture supernatants and intracellular viral protein levels in a dose-dependent manner. Here, we suggest that tonsil epithelial organoids could provide a preclinical and translational research platform for investigating SARS-CoV-2 infectivity and transmissibility or for evaluating antiviral candidates.


Keywords: Antiviral; SARS-CoV-2; Tonsil epithelial organoid; Tonsil tissue; Transcriptome.
- PMID: 35286852


- Fulltext: https://www.sciencedirect.com/science/article/pii/S0142961222000990?via%3Dihub